Isolation and Identification of Haemophilus parasuis SD02 Strain

摘要

A bacterial strain was isolated from the sick pigs suspiciously infected by polyserositis and arthritis in a pig farm in Shandong Province,and identified through morphological observation,culture traits,biochemical characteristics and PCR amplification.Additionally,primers were de-signed according to the 16S rRNA sequence of Haemophilus parasuis,and the bacterial strain was amplified by PCR.The amplified fragments of approximately 1 400 bp was sequenced,and aligned with the sequence in Gen Bank.The results showed that it shared the homology of 97%-99%with the 16S rRNA sequence of foreign H.parasuis,and confirmed as H.parasuis(HPS).The strain was determined as serotype 4 through serotype identification.The strain was named SD02.