连翘提取物对常见致病菌的体内外抑菌活性测定

摘要

In order to observe the antibacterial activity of Forsythia suspense extract on Staphylococcus aureus, Escherichia coli, Salmonella sp. and other pathogens, the antibacterial activities of F. suspensa extract on S. aureus, E. coli and Salmonella sp. in vitro were determined by using tube double-dilution method. After mice were administrated with the same concentrations of pathogens by intragastric administration, mice were treated with F. suspensa extract to determine the number of bacteria in the duodenum, jejunum, ileum and cecum of mice. The results showed that F. suspensa extract had inhibitory effects on S. aureus, E. coli and Salmonella sp. in vitro and in vivo. And the inhibitory effect on S. aureus was the strongest and that on Salmonella sp. was the weakest. The mortality of mice induced by pathogens was significantly reduced by perfusing F. suspensa extract. In conclusion, F. suspensa extract had obvious inhibitory effects on S. aureus, E. coli, Salmonella sp. and other common pathogens in vivo and in vitro.%为观察连翘提取物对金黄色葡萄球菌、大肠杆菌和沙门氏菌等致病菌的抑菌活性，试验采用试管二倍稀释法，测定连翘提取物对金黄色葡萄球菌、大肠杆菌和沙门氏菌的体外抑菌活性；采用相同浓度致病菌灌胃小鼠后，分别用连翘提取物灌胃治疗，测定小鼠十二指肠、空肠、回肠和盲肠中肠道菌群数量。结果表明，无论是在体外还是在体内，连翘提取物对金黄色葡萄球菌、大肠杆菌和沙门氏菌均具有抑制作用，且对金黄色葡萄球菌抑制性最强，对沙门氏菌抑制性最弱；通过灌注连翘提取物，显著降低了各致病菌致病后小鼠的死亡率。通过研究得出以下结论，连翘提取物对常见的金黄色葡萄球菌、大肠杆菌和沙门氏菌等致病菌具有较明显的体内外抑菌作用。