外源性壳聚糖对鲤鱼体内部分组织中壳聚糖沉积量的影响研究

摘要

为了解饲喂壳聚糖后鲤鱼体内部分组织中壳聚糖的沉积情况，将210尾鲤鱼随机分为7组，即对照组和试验Ⅰ～Ⅵ组。试验Ⅰ～Ⅵ组饲料中分别添加0.50%、1.00%、1.50%、2.00%、3.00%、4.00%的壳聚糖，对照组饲喂常规饲料。正试期60 d，每隔10 d 分别从各试验组及对照组的每个重复中随机选取1尾鲤鱼，测定并比较不同处理组鲤鱼鱼鳞、鱼鳍、鱼肌肉组织中的壳聚糖沉积量。结果表明，随着养殖时间的延长和饲料中外源性壳聚糖添加量的增加，对照组和所有试验组鲤鱼的鱼鳞、鱼鳍、鱼肌肉组织中的壳聚糖沉积量均有不同程度的提高。但当饲料中外源性壳聚糖的添加量≥3.00%时，鱼鳞中壳聚糖沉积量的变化不显著（P＞0.05）；当添加量≥1.50%时，鱼鳍中壳聚糖沉积量的增加差异不显著（P＞0.05）；在相同的试验阶段，各试验组与对照组之间、各试验组之间鱼肌肉组织中壳聚糖的沉积量差异均不显著（P＞0.05）。结果提示，当饲料中外源性壳聚糖的添加量达到一定程度时，鱼鳞及鱼鳍中壳聚糖的沉积量不会随着添加量的增加而显著增加，且添加不同水平的外源性壳聚糖，对鲤鱼肌肉组织中壳聚糖沉积量的影响较小。%In order to determine the chitosan deposit level in selected tissues of carp after they fed with exogenous chitosan, a total of 210 carp were randomly divided into 7 groups, including control group and experimental groups Ⅰ-Ⅵ. The basic diet of experimental groupsⅠ-Ⅵwere added with 0.50%、1.00%、1.50%、2.00%、3.00%and 4.00%chitosan, respectively, the control group fed with routine diet without exogenous chitosan adding. During the 60 d of formal test period, one carp was randomly selected in each replicate from the control group and experimental groups every 10 d respectively to determine the chitosan deposit level in scale, fin and myoideum. The results showed that with the extension of feeding time and the increase of adding amount of exogenous chitosan in feed, the chitosan deposit level in all tested tissues for control group and experimental groups were increased in different degrees. However, no significant differences were observed in the change of chitosan deposit level in scale when the adding amount of exogenous chitosan in feed exceeded 3.00%, and there were no significant differences of the increase of chitosan deposit level in fin when the adding amount of exogenous chitosan in feed exceeded 1.50%.In the same experimental period, there were no significant differences of chitosan deposit level in myoideum between control groups and experimental groups or among the experimental groups. It was indicated that when the adding amount of exogenous chitosan in feed reached certain degree, the chitosan deposit level in scale and fin would not significantly increase with the increase of adding amount. Furthermore, the chitosan deposit level in myoideum was less influenced by exogenous chitosan in feed with different adding amount.