乌头提取物对心衰大鼠抗氧化能力的研究

摘要

Objective To investigate the effect of aconite extract on antioxidant capacity in rats with heart failure and the role of aconite extract in treatment of heart failure.Methods Ninety Wistar rats were randomized into three groups, blank control group(n=30), control group(n=30) and experimental group(n=30).The experimental group and the control group were abdominal aortic coarcta-tion rat model of chronic heart failure.After modeling the control group received injections of digoxin 20 mg· kg-1 , once a day for 28 days of continuous intervention, the experimental group had an additional use of aconite extract, administered 0.04 mg· kg-1 for an intervention of 28 days and the blank control group had tail vein injection of the same volume of satine at 0.04 g· kg-1 .After the end of the intervention, the rats were anesthetized by intraperitoneal injection, abdominal aortic blood taken from the upper serum after cen-trifugation.Kit method was used to measure superoxide dismutase ( SOD) , malondialdehyde ( MDA) and reduced glutathione ( GSH) . Results After the modelling, SOD, MDA and GSH levels in the experimental group and the control group were significantly lower than the blank control group with statistical difference (P<0.05).SOD, MDA and GSH levels in experimental group and control group were not significantly different (P>0.05).Seven days and 28 days after intervention, compared with the blank control group, SOD, MDA and GSH levels in the experimental group and control group were significantly increased with statistically difference ( P<0.05).Compared with the control group, SOD, MDA and GSH levels in the experimental group were significantly increased with sta-tistical difference (P <0.05).Conclusion Aconitum extract injected into the body of rats with heart failure can promote the body's antioxidant capacity and improve cardiac dysfunction, which is worthy of promotion.%目的：探讨乌头提取物对心衰大鼠抗氧化能力，并明确乌头提取物治疗心衰的作用。方法选取90只Wistar大鼠随机分为3组，空白组30只，对照组30只及实验组30只。实验组和对照组采用腹主动脉缩窄法制作慢性心衰大鼠模型。造模成功后对照组给予注射地高辛20 mg· kg－1，连续干预28 d；实验组在对照组的基础上加用乌头提取物，灌胃0．04 mg· kg－1，干预28 d，空白组以等体积生理盐水尾静脉注射0．04 g· kg－1。干预结束后，对大鼠采用腹腔注射麻醉方法，取腹主动脉血离心后取上层血清，按试剂盒方法测定超氧化物歧化酶（ SOD）、丙二醛（ MDA）、还原型谷胱甘肽（ GSH）。结果造模结束后，实验组与对照组SOD、MDA与GSH水平明显低于空白组，差异有统计学意义（ P＜0．05）。实验组与对照组SOD、MDA与GSH水平无明显差别，（ P＞0．05）；干预7 d及28 d后，与空白组相比，实验组与对照组SOD、MDA与GSH水平明显升高，差异有统计学意义（P＜0．05），与对照组相比，实验组SOD、MDA与GSH水平明显升高，差异有统计学意义（P＜0．05）。结论向心衰大鼠体内注射乌头提取物，对提高心衰机体的抗氧化能力、改善心功能不全指导意义，值得临床推广。