高效液相色谱法测定复方枇杷喷托维林颗粒中枸橼酸喷托维林的含量

摘要

Objective To establish a HPLC method for the determination of pentoryverine citrate in Compound Loquat and Pentoryverine Granules.Methods The chromatorgraphic conditions were as follows:Aglient C18 (150 mm×4.6 mm,5 μm)column was used.1%triethylamine(pH 3.0)-acetonitrile(70∶30) was used as mobile phase,and the flow rate was at 1.0 mL·min-1.The detect wavelength was at 210 nm,the column temperature was 35 ℃.Results Thecalibration curves were linearly in the range of 0.02-1.01 g·L-1,the regression equation was Y=6 652 832.11 X-8 399.71(r=0.999 9).The average recover was 99.4%.Conclusion The method was simple,accurateand can be used for quality control.%目的 建立复方枇杷喷托维林颗粒中枸橼酸喷托维林含量的高效液相测定方法.方法 采用Aglient C18(150 mm×4.6 mm,5 μm)色谱柱;流动相:1%三乙胺溶液(用磷酸调pH至3.0)-乙腈(70∶30);流速:1.0 mL·min-1;检测波长:210 nm.结果 枸橼酸喷托维林在0.02~1.01 g·L-1的质量浓度范围内线性关系良好,Y=6 652 832.11 X-8 399.71(r=0.999 9),平均加样回收率为99.4%,RSD=1.2%.结论 该法简便、准确,可用于复方枇杷喷托维林颗粒中枸橼酸喷托维林的质量控制.