链霉素抗性筛选吸水链霉菌高产农用抗生素菌株研究

摘要

[Objective]The research aimed to screen Streptomyces hygroscopicus strains with high production of agricultural antibiotics.[Method]A strain of S.hygroscopicus was screened from the soil of Hainan Island.After natural screening and consecutive uhravidet induced mutation twice,S6-7 strain was obtained as the original strain then treated by UV irradiation and streptomycin resistance screening,and finally rescreened through shake-flask fermentation.[Result]7 better strains were selected by primary screening from 62 single colonies which were picked out randomly.After 3 generations of consecutive cultivation on slant media and rescreening,5 strains presented obvious forward mutation.The forward mutation rate reached 8.06%,and the largest production increasing rate came up to 25.11%.[Conclusion]By combining streptomycin resistance screening and conventional uhraviolet induced mutation,both the antibiotic-producing capacity and forward mutation screening efficiency of the original strain were greatly enhanced.