[Objective] This study aimed to investigate rapid multiplication of Apocynum by tissue culture so as to provide plantlet sources for its industrialized cultivation. [Method] The asepsis seedlings were obtained by dealing with Apocynum seeds. Its cotyledons, hypocotyls and shoot tips were cultured on the media containing different concentrations of hormones. Finally, the influence of different hormone combinations on differentiation of cotyledons and hypocotyls, rapid multiplication of shoot tips, rapid multiplication of regenerated shoots, and rooting of test-tube plantlets was com- pared. [Result] MS+2.0 mg/L BA+0.03 mg/L NAA and MS+0.07 mg/L NAA were the optimum medium for inducing regenerated buds from cotyledons and hypocotyls re- spectively; MS+2.0 mg/L BA+0.02 mg/L NAA was the best medium for rapid multi- plication of shoot tips; MS+1.9 mg/L BA+I.7 mg/L NAA was the best medium for rapid multiplication of regenerated buds: and 1/2MS+0.6 mg/L NAA was the best medium for inducing roots. [Conclusion] The optimum hormone combination was de- termined for Apocynum rapid multiplication by tissue culture, which provides technical support on Apocynum industrialized cultivation.
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机译：［目的］研究罗布麻通过组织培养的快速繁殖，为小罗布麻的工业化生产提供植物来源。 ［方法］通过处理罗布麻种子获得无菌苗。在含有不同浓度激素的培养基上培养其子叶，下胚轴和芽梢。最后，比较了不同激素组合对子叶和下胚轴分化，芽尖快速繁殖，再生芽快速繁殖以及试管苗生根的影响。 [结果] MS + 2.0 mg / L BA + 0.03 mg / L NAA和MS + 0.07 mg / L NAA分别是诱导子叶和下胚轴再生芽的最佳培养基。 MS + 2.0 mg / L BA + 0.02 mg / L NAA是快速繁殖枝条的最佳培养基。 MS + 1.9 mg / L BA + 1.7 mg / L NAA是再生芽快速繁殖的最佳培养基：1 / 2MS + 0.6 mg / L NAA是诱导根的最佳培养基。 [结论]通过组织培养确定了罗布麻快速繁殖的最佳激素组合，为罗布麻工业化栽培提供了技术支持。
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