The aim of this study was to investigate the in vitro antifungal effects of antifungal monomer component DZP8 isolated from Streptomyces 702 on the mycelium growth, sclerotium formation and germination of Rhizoctonia solani and on the mycelium growth, conidial formation, germination, appressorium formation of Magnaporthe grisea. The results showed that the antifungal monomer component DZP8 has strong antifungal effect on both the R. solani and M. grisea. The EC50 and EC90 of DZP8 were 1.81 and 3.35 μg/ml on Ft. solani respectively, and 37.01 and 136.21 μg/ml on M. grisea respectively. Under the treatment of 48.01 μg/ml DZP8, the sclerotium formation rate of R. solani was just 39.21%, the formation time delayed by 216 h and the dry weight decreased by 81.37% in comparison the con- trol; and 33.51 μg/ml DZP8 significantly inhibited the sclerotium germination. In the presence of 160.08 μg/ml DZP8, the sporulation of M. grisea was just 9.29% of control sample; 20.14 μg/ml DZP8 inhibited the conidial germination suppression rate by 95.16%, and the appressorium formation by 100%.
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机译:这项研究的目的是调查从链霉菌702分离的抗真菌单体成分DZP8在体外对Rhizoctonia solani的菌丝生长,菌核形成和萌发以及对Magnaporthe grisea的菌丝生长,分生孢子形成,萌发,贴壁形成的体外抗真菌作用。 。结果表明,抗真菌单体成分DZP8对茄形梭菌和稻瘟病菌均具有较强的抗真菌作用。在Ft上DZP8的EC50和EC90为1.81和3.35μg/ ml。分别为solani和grisea grisea上的37.01和136.21μg/ ml。与对照相比,在48.01μg/ ml DZP8处理下,茄形假单胞菌的菌核形成率仅为39.21%,形成时间延迟了216 h,干重降低了81.37%。 33.51μg/ ml DZP8显着抑制菌核的萌发。在160.08μg/ ml DZP8的存在下,稻瘟病菌的孢子形成仅占对照样品的9.29%; 20.14μg/ ml的DZP8抑制分生孢子萌发抑制率达95.16%,抑制ress的形成达100%。
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