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Genetic Analysis of Genomic DNA in Shanxi Lean Meat Pigs (SD-Ⅲ Line)

机译:山西瘦肉猪(SD-Ⅲ系)基因组DNA的遗传分析

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摘要

Abstract [Objective] This study aimed to detect the purity of Shanxi lean meat pigs. [Method] Eight primers were selected and used in the DNA fingerprinting technique amplified fragment length polymorphism (AFLP) to detect the purity of 30 Shanxi lean meat pigs. [Result] A total of 206 AFLP markers were obtained; 3-15 bands were obtained from each primer. The similarity index of population in Shanxi local pigs was 0.931 on average. [Conclusion] AFLP is suitable for detection of genomic DNA, and the genetic purity of SD-Ⅲ line is higher.
机译:摘要[目的]研究山西瘦肉猪的纯度。 [方法]选择8条引物,用于DNA指纹图谱扩增片段长度多态性(AFLP)检测30只山西瘦肉猪的纯度。 [结果]共获得206个AFLP标记。从每个引物获得3-15条带。山西当地生猪的种群相似指数平均为0.931。 [结论] AFLP适合于基因组DNA的检测,SD-Ⅲ系的遗传纯度较高。

著录项

  • 来源
    《农业科学与技术(英文版)》 |2012年第7期|1579-1582|共4页
  • 作者单位

    Key Lab of Preventive Veterinary Medicine in Hebei Province, College of Animal Science and Technology, Hebei Normal Uni- versity of Science and Technology, Qinhuangdao 066600, China;

    Vocational and Technical Education Center in Nanhe County, Nanhe 054400, China;

    Key Lab of Preventive Veterinary Medicine in Hebei Province, College of Animal Science and Technology, Hebei Normal Uni- versity of Science and Technology, Qinhuangdao 066600, China;

    Key Lab of Preventive Veterinary Medicine in Hebei Province, College of Animal Science and Technology, Hebei Normal Uni- versity of Science and Technology, Qinhuangdao 066600, China;

    Key Lab of Preventive Veterinary Medicine in Hebei Province, College of Animal Science and Technology, Hebei Normal Uni- versity of Science and Technology, Qinhuangdao 066600, China;

    College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China;

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  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 农业科学;
  • 关键词

    Key; words; Pigs; Genomic; DNA; AFLP; Molecular; marker;

    机译:关键词;猪;基因组;DNA;AFLP;分子;标记;
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