斑马肾上腺的组织学研究

摘要

In order to explore the histological structure of zebra and to protect endangered species diversity,it is especially important to study the zebra adrenal histological structure.2 zebra adrenals were studied by means of histological methods.The adrenal gland tissues were dissected into serial paraffin sections at the thickness of 3 μm.After HE and Masson staining,they were observed and photographed by the light microscope and Image-Pro Plus 6.0.The results show that zebra adrenal capsule is covered with thick connective tissue,which is about 230 μm.A large number of nerves and blood vessels are distributed in the capsule.The area of cortex is about 5 times larger than medullae.Zona glomerulosa is thin,about 300?m.The cells were arranged as column and ball.Zona fasciculata with the thickness of 1 750 μm is 6 times as thick as zona glomerulosa.The cells were arranged in cord.Zona reticular with the thickness of 250 μm was closely adjacent to medulla and formed a jagged boundary.The medulla with the area of 4 mm2 was deeply dyed.It was concluded that some sympathetic ganglion cells were found in the medulla arranged in groups and surrounded with zona reticular cells but lots of the cells were found in zona reticular.Some connective tissue with chromaffin cells was found in the medulla center vein.The purpose of the present study provides reliable evidences for further study on morphology,physiology and pathology of the zebra.%探明斑马肾上腺的组织学结构,为深入研究斑马的生理学、病理学和保护濒危物种的生物多样性提供形态学依据.采用石蜡切片,HE染色和Masson染色技术,显微摄影,以及图像分析技术,与家畜的肾上腺进行比较,研究了斑马肾上腺的组织结构特点.结果显示斑马肾上腺具有以下特点:表面结缔组织被膜较厚,平均厚度大约230 μm.被膜内有大量神经和血管分布.皮质约为髓质面积的5倍.皮质球状带厚度大约300 μm,比较薄,细胞排列成柱状、团块状；束状带厚度1 750μm左右,约为球状带的6倍,细胞排列成条索状；网状带厚度250 μm左右,网状带细胞与髓质紧密相邻并与之形成参差不齐的锯齿状界限；网状带中含有神经元.斑马髓质染色比较深,面积大约4 mm2,髓质细胞比较大,胞质嗜碱性.髓质中未见到交感神经节细胞；髓质中央静脉内壁局部发现有结缔组织,且其中含有神经元.该研究结果为深入研究斑马的形态学、生理学和病理学提供可靠的形态学依据.