A Few Questions that Should be Clarified in the Polymerase Chain Reaction

摘要

The polymerase chain reaction is one of the most useful technical advance and inventions in modern molecular biology. Developed in 1983 by Kary Mullis[1], PCR is now a common and indispensable technique used in medical and biology research labs for a variety of applications[2-3]. A large number of articles related to PCR are available on the internet and other places. People know well about the basic principle and are very familiar with the procedures of the PCR. But, some details were neglected on the numbers of the target sequence and other DNA strands number after 30 to 35 cycles of the PCR. In most papers, the number of newly synthesized DNA strands including target DNA and non target DNA is ambiguous and even wrong. In this paper, highlights were given to the theoretical number of target DNA number in details and the exact number of the target DNA number can be concluded by analysis.