Changes of NF-κB, Bax and Caspase 3 in Apoptosis lnduced by Ligustrazine Combined with Cis-dichlorodiamine Platinum in Human Gastric Carcinoma SGC-7901 Cell Lines

摘要

Objective] This study aimed to investigate the mechanism of apoptosis in-duced by ligustrazine (TMP) and cis-dichlorodiamine platinum (DDP) in SGC-7901 cel lines in vitro. [Methods] SGC-7901 cel lines were treated with ligustrazine and DDP alone or combined for 48 h for Western blot analysis, respectively. Western blot analysis was used to determine the expression of proteins involved in apoptosis including NF-κB p65, bax and caspase-3. [Results] The viability of SGC-7901 cel s was inhibited after treated with ligustrazine and/or combined with DDP. The expres-sion of NF-κB P65 protein decreased after treated with drugs, in which the protein decreased significantly in 1.2 mg/ml of TMP combined with 2 μg/ml of DDP group. Meanwhile, we investigated the protein expression of bax and caspase-3. The re-sults showed that the expression of the two proteins increased fol owing with the in-creasing concentration of TMP. [Conclusion] Al the results indicated that ligustrazine combined with DDP could induce the apoptosis of SGC-7901 cel lines, and NF-κB maybe the possible way to induce the cel apoptosis.