糖种类和浓度及pH值对长白山笃斯越桔组培苗增殖和生根的影响

摘要

In vitro shoots of Vaccinium uliginosum were used as experimental material to study effect of sugar types,concentrations and pH value on proliferation and rooting of Vaccinium uliginosum in vitro.The results showed that 30 g/L sucrose concentration are suited for Vaccinium uliginosum subculture multiplication and the root,the propagation coefficient reached 5.3 times and rooting rate of over 80%;pH 4.2 is the most suitable for Vaccinium uliginosum subculture multiplication and the root,the propagation coefficient reached 5.9 times and rooting rate of over 80%.%以长白山笃斯越桔组培苗为试材,研究糖及pH值对笃斯越桔组培苗继代增殖及生根的影响,结果表明,30g/L的蔗糖浓度适宜于继代和生根,继代培养30d后的增殖倍数可达5.7倍,生根培养50d后的生根率可达80%;pH值4.2的培养基适宜笃斯越桔继代增殖及生根,继代培养30d后的增殖倍数可达5.9倍,生根培养50d后的生根率可达80%。