滁菊总黄酮的镇痛作用及与一氧化氮和前列腺素E2的关系

摘要

目的研究滁菊总黄酮( TFCC)的镇痛作用及其可能机制。方法镇痛作用采用小鼠扭体法、温浴法、福尔马林法、热板法进行检测；血清和脑组织中一氧化氮( NO)和前列腺素E2( PGE2)含量分别采用Griess法和紫外分光光度法测定。结果灌胃给药TFCC 100、200 mg/kg可明显减少小鼠扭体反应数，降低小鼠福尔马林致痛作用第Ⅱ时相疼痛反应评分值，延长小鼠热板舔足反应潜伏期和热水缩尾反应潜伏期；小鼠侧脑室给药TFCC 5、10 mg/kg可明显抑制小鼠扭体反应。灌胃给药TFCC 100、200 mg/kg可明显提高小鼠血清和脑组织中NO含量，但可降低PGE2含量。结论 TFCC具有明显镇痛作用，其镇痛机制可能与促进 NO 释放和抑制PGE2生成有关。%Objective To study the analgesic effect of total flavone of Chuzhou chrysanthemum ( TFCC ) and its mechanism. Methods Writhing test, warm bath method, formalin method, the hot plate test were used to examine analgesic effect on mice, and serum and brain tissue nitric oxide( NO) and prostaglandin E2 ( PGE2 ) contents were determinated the Griess method and ultraviolet spectrophotometry. Results Intragastrical administration of TFCC 100 mg/kg and 200 mg/kg could significantly reduce the writhing number in mice,II-phase pain in formaldehyde induced pain in mice, and prolonged the mice hot plate licking foot response latency and hot shrinkage tail reaction time. Intracerebroventricular injection TFCC 5 mg/kg and 10 mg/kg could significantly inhibit the writhing number in mice. TFCC 100 mg/kg and 200 mg/kg significantly increased mice serum and brain NO contents, and reduced PGE2 contents. Conclusion TFCC has obvious analgesic effect, and its analgesic mechanism may be related to the promation of NO release and the inhibition of PGE2 .