诱导烟草早花的PVX-FT系统的建立

摘要

FT is a key gene controlling plantflowering. Exogenous genes could be transiently expressed in high level by PVX vector. PVX transient expression vector of FT gene from Arabidopsis was constructed. Different types of tobacco were infected using agrobacterium infiltration. Results showed that, PVX-FT induced earlyflowering influe-cured tobacco variety K326, Yunyan87 and Honghuadajinyuan, oriental tobacco variety TEVB, and burley tobacco variety TN90. It is suggested that FT gene can be used in tobacco breeding.%FT(Flowering locus T)基因是控制植物开花的关键基因，而PVX(Potato virus X)载体能够使外源基因在植物体内高水平瞬时表达。本文构建了拟南芥FT基因PVX病毒瞬时表达载体，通过农杆菌渗滤感染不同类型烟草品种，使拟南芥FT基因在烟草中瞬时表达。结果表明，该系统能够诱导烤烟品种K326、云烟87和红花大金元，香料烟品种TEVB及白肋烟品种TN90早花，应用于烟草育种，可缩短育种进程。