GC-MS/O技术测定卷烟烟气中巨豆三烯酮的香气活力值

摘要

以同时蒸馏萃取法萃取卷烟主流烟气粒相物,利用半制备高效液相色谱法制备含有巨豆三烯酮馏分,采用GC-MS/O技术测定巨豆三烯酮4种同分异构体在卷烟烟气中的含量和嗅觉阈值,从而获得了相应香味化合物的香气活力值(OAV).结果表明:①巨豆三烯酮a、b、c和d的嗅觉阈值分别为0.81 ng/mL、2.05 ng/mL、3.86 ng/mL和0.88 ng/mL;②巨豆三烯酮4种同分异构体的OAV大小顺序为:巨豆三烯酮d(75.58)＞巨豆三烯酮b(35.35)＞巨豆三烯酮a(23.01)＞巨豆三烯酮c(2.36);③利用GC-MS/O技术,可获得化学性质不稳定或存在多种同分异构体的香味物质OAV,解决了传统方法无法准确测定的问题.%Simultaneous distillation and extraction was applied to handle cigarette mainstream smoke particles,and fractions containing megastigrnatrienone were prepared using Semi-preparation HPLC.Contents and odor threshold values of megastigmatrienone in tobacco smoke were determined by GC-MS/O respectively,and odor active values (OAV) were obtained.Results showed that:1) Odor threshold value of four isomers of megastigmatrienone was 0.81 ng/Ml,2.05 ng/mL,3.86 ng/mL and 0.88 ng/mL respectively.2) OAVs of four isomers of megastigmatrienone were in the order of megastigmatrienone d (75.58) ＞ megastigmatrienone b (35.35) ＞ megastigmatrienone a (23.01) ＞ megastigmatrienone c (2.36).3) Odor threshold values of flavoring substances which possessed unstable chemical properties or several isomers could be determined accurately by using GC-MS/O technology,and hence the odor active values (OAV) in the cigarette smoke.The technology boasted accurate determination of OAVs of flavoring substances compared with traditional methods.