A novel method for investigating traffic tolerance of turf grass was built on the basis of the invention of a portable traffic simulator and a new approach for traffic treatment. Four traffic treatments (non-traffic, light traffic, moderate traffic and severe traffic) were used in the method for the experiment of traffic tolerance of perennial ryegrass (Lolium perenne) , and five indexes, including visual rating, coverage, chlorophyll content, leaf water content and fresh weight of aboveground biomass of turf grass, were measured at 1, 3, 7, 10, 14 and 21 day after traffic treatments. Results showed that five indexes of perennial ryegrass showed different changes under different degree of traffic stress. The value of all indexes declined obviously with increasing intensity of traffic under all kinds of traffic treatments except that chlorophyll content was higher than CK (non-traffic) under light traffic. Values of all indexes began to increase after 3 days from traffic treatment and kept rising with increasing days. Under light traffic and moderate traffic, the value of all indexes reached to CK level at 7 d and 14 d after traffic treatments, respectively. However, the value of all indexes under severe traffic did not reach to CK level at 21 d after traffic treatments. Correlation between visual rating and coverage, chlorophyll content, leaf water content and fresh weight of aboveground biomass were analyzed and highly close relationships were found at 7 d after traffic treatments (P<0. 01). The correlation coefficients were the highest at 14 d after traffic treatments. Therefore, 14 days after traffic treatments may be an important period for physiological regulation of perennial ryegrass in response to traffic stress. The results can provide theoretical basis for further in-depth research about the mechanism of traffic tolerance of turf grass.%为推动草坪草耐践踏机理的研究,自行设计了一款便携式草坪践踏器,提出了一套新的草坪践踏处理方法 ——集中践踏,从而建立了一种新的草坪草耐践踏试验方法.运用该试验方法,通过设定4个践踏处理(不践踏、轻度践踏、中度践踏和重度践踏),对多年生黑麦草进行耐践踏试验,测定了践踏后1,3,7,10,14和21 d的草坪表观质量、盖度、叶绿素含量、叶片含水量和地上生物量鲜重等5项指标.结果表明,在不同的践踏强度下,多年生黑麦草的各项指标呈现出不同的变化趋势,除叶绿素含量在轻度践踏下比CK(不践踏)略微升高外,其他指标均随践踏强度的增加而明显降低.在践踏后3d及以后,所有指标均随时间的增加而呈上升趋势.轻度践踏下,各项指标在践踏后7d恢复到接近CK的水平；中度践踏下,各项指标在践踏后14d恢复到接近CK的水平,而重度践踏下,各项指标在21 d内均未恢复到CK的水平.将草坪表观质量与盖度、叶绿素含量、叶片含水量和地上生物量进行了相关性分析发现,两两之间均存在高度相关性,特别是在践踏后7d及以后,其相关系数均达到了极显著水平,而且相关系数在践踏后14d达到最高.因此认为,多年生黑麦草在践踏后的14 d内,可能是其生理上响应践踏胁迫的关键时期.该研究结果为进一步深入研究草坪草耐践踏机理提供了理论基础.
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