嗜盐古菌染色体DNA片段在大肠杆菌中的启动子功能

摘要

A promoter-probe plasmid pKK232-8 was used as a vector, which functioned in eubacteria Escherichia(E.) coli HB101. The promoter function of the chromosome DNA fragment from Halobacterium(H.) halobium R1, RM13 DNA fragment, active as eubacteria promoters in E.coli HB101,was studied by microcalorimetry.The promoter is of about 1 000 bp(base pair).It can promote the chloramphenicol acetyltransferase (CAT) gene in plasmid pKK232-8,and the chloramphenicol resistance level is about 150 mg@ L- 1.The result indicates that there are probably double-function promoters in H.halobium chromosome.%以启动子探针质粒 pKK232-8为载体 ,用微量量热法研究了源自盐生盐杆菌 R1染色体的 RM13 DNA片段在大肠杆菌 HB101中的真细菌启动子功能 ,该启动子 RM13 DNA片段的大小为 1000bp(碱基对 ),它能启动探针质粒 pKK232-8上的氯霉素乙酰水解酶基因 (即 :氯霉素抗性基因 Cmr),氯霉素抗性水平可达到 150 mg@ L- 1,抗性水平较高 ,启动活性较大 .研究结果表明 ,在盐生盐杆菌染色体上可能存在具有双功能或多功能的启动子 DNA片段 ,这对系统发育、微生物遗传学和生物热化学等均有重要的意义.