Huperzine A derivative M3 protects PC12 cells against sodium nitroprusside-induced apoptosis

摘要

To investigate the effects of M3,a derivative of huperzine A,on the apoptosis induced by sodium nitroprusside (SNP) in PC12 cells.Methods:Cell viability was detected using MTr method.Apoptosis was examined with annexin V/prodium iodide (PI) stain.The levels of reactive oxygen species (ROS) were measured using fluorophotometric quantitation.The amount of malonaldehyde (MDA) was determined with MDA detection kits.The expression of caspase-3 and Hsp70 were analyzed using Western blotting.Results:Exposure of PC12 cells to SNP (200 μmol/L) for 24 h decreased the cell viability to 69.0％ of that in the control group.Pretreatment with M3 (10 μmol/L) or huperzine A (10 μmol/L) significantly protected the cells against SNP-induced injury and apoptosis; the ratio of apoptotic bodies in PC12 cells was decreased from 27.3％ to 15.0％.Pretreatment with M3 (10 μmol/L) significantly decreased ROS and MDA levels,and increased the expression of Hsp70 in the cells.Quercetin (10 μmol/L) blocked the protective effect of M3,while did not influence on that of huperzine A.Conclusion:M3 protects PC12 cells against SNP-induced apoptosis,possible due to ROS scavenging and Hsp70 induction.