Appling YAG double-frequency laser microbeam of different energy irradiated four cell stage embryos of Bufo bufo gargarizans. The early stage embryos' development was observed and the results show that low energic saser could make hatching speed rise and less abnormal embryos, while high energic laser could hold back embryos' development and make more embryos abnormal. The patterns of protein expression of early blastula were investigated by two-dimensional electrophoresis 0.5 hour after irradiation with different energic saser microbeam and PDQuest software was used to examine protein spots' distribution during different pI and molecular weight in protein gel after scanned by GS-800 protein densitometers of Bio-Rad. The results show that protein exdpression was influenced by laser irradiation and total protein spots were related to energy densities of laser microbeam.%应用YAG倍频激光器选择不同能量密度激光微束对中华大蟾蜍4细胞期胚胎进行照射,观察胚胎的生长、发育状况,统计出膜率、畸胎率和死亡率.实验结果表明:低能量激光照射明显地提高了胚胎的孵化速度,且使畸变率降低;而高能量激光对胚胎发育有明显抑制作用,而且使畸变率增高.不同能量密度激光微束照射中华大蟾蜍早囊胚,0.5 h后制备样品进行双向电泳,凝胶成像后用PDQuest软件进行分析.结果显示激光照射对蛋白质点数及其分布有影响,影响程度与照射激光密度有关.结论:激光照射可以影响胚胎发育和蛋白质的合成.
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