表面引发的DNA杂交链式反应的石英晶体微天平研究

摘要

The hybridization chain reaction (HCR), a type of nucleic acid amplification method, was studied by a quartz crystal resonator technique. We probed the properties of the boundary DNA layer with a thickness of the viscous penetration depth at the crystal-solution interface. According to the relationship between the change of resonant frequency and the mass adsorbed on the crystal surface, the kinetics of surface-initiated HCR was measured, as well as the viscosity and the shear modulus of H1/H2 solution with different concentrations near a solid substrate. This work provides a new method for study- ing nucleic acid reactions at solid surface and mechanistic insight for HCR-based amplification and biodetection.%通过石英晶体振荡技术研究了杂交链式反应这种核酸扩增的方法。 石英晶体微天平可以表征在晶体和溶液的界面上的DNA层, 并获得粘性穿透深度这一重要参数。 根据石英晶体表面吸附质量和振荡频率之间的关系, 我们测量了表面引发的杂交链式反应的动力学过程, 并获得界面上的粘度、剪切模量等参数。 这一工作为研究固液界面上核酸反应过程, 特别是杂交链式反应的机制提供了新的途径。