质谱成像及其在生物医学领域的应用

摘要

质谱成像(mass spectrometry imaging,MSI)是一种研究生物组织或细胞中分子组成及分布的新型分析技术,可在无标记的情况下同时检测并记录样本表面多种分子的空间分布信息.MSI包括4个基本步骤:样本准备、离子化、分子的质量分析及图像重构.目前,该技术已被广泛应用于研究组织中元素、代谢物、蛋白质和药物及其代谢物等的分布.本文介绍了MSI的原理、离子化技术和技术流程,并综述了MSI在生物医学领域中的应用情况.%Mass spectrometry imaging (MSI) is an innovative molecular analytical technique that combines th< chemical and spatial analyses of surface. It also simultaneously allows spatial localization of multiple differen compounds; such as elements; metabolites; proteins and drug and its metabolites; without label steps. Process of MSI involves sample preparation; molecular ionization; mass analysis and image reconstruction This review described the mechanism; ionization techniques; workflows and application areas of MSI.