Expression,purification,crystallization,and preliminary X-ray diffraction analysis of the human TLE1 Q domain

摘要

Human transducin-like enhancer of split 1(TLE1)plays crucial roles in a number of developmental processes and is involved in pathogenesis of malignancy tumors.The N-terminal glutamine-rich domain(Q domain)of TLEI mediates its tetramerization and interactions with different DNA-binding transcription factors to regulate Notch and Wnt signaling pathways.To better understand the molecular mechanism of TLEI's functions in these pathways,we cloned,purified,and crystallized the TLE1 Q domain(TLEI-Q).The crystals belong to space group C2221,with the complete diffraction data of the native and Se-Met TLEI-Q collected to 3.5 and 4.1 A resolutions,respectively.The phasing-solving and model building are in progress.