AFLP分子标记在玉米优良自交系优势群划分中的应用

摘要

AFLP marker technique has been used to study the diversity of 17 elite maize inbred lines. Four AFLP primer combinations revealed 30, 30, 44 and 41 AFLP Polymorph bands, with an average of 36.25 bands produced per primer pair. Using 4 primer combinations that were able to discriminate between genotypes could produce 145 AFLP bands. The cluster analysis based on AFLP data showed that 17 elite maize inbred lines could be divided into 6 groups accorded with pedigree, AFLP could be used to study the genetic diversity of maize inbred lines and to group maize inbred lines.%本文利用AFLP分子标记技术研究了17个玉米优良自交系的遗传多样性，4个AFLP引物组合分别扩增出30、30、44、41条多态性带，严均每个引物组合扩出36．25条带，4个引物组合共扩增出145条带，每一个引物组合都可将17个自交系完全分开。利用AFLP数据、进行聚类分析，将17个优良自交系聚为6群，结果表明，用AFLP标记进行玉米优势群划分与自交系系谱亲缘关系基本一致，AFLP技术可以用于玉米自交系的遗传多样性研究和优势群划分。