金柑童期短,1年可多次开花结果,有利于通过遗传转化开展花、果性状和相关基因在花、果实中表达的研究,但目前金柑实生苗再生率还有待提高。以广西阳朔的金弹金柑实生苗节间茎段为材料,发现以35 d苗龄(暗培养25 d,光照10 d)的实生苗节间茎段为外植体材料,培养于再生培养基( MS+3 mg/L 6-BA)上50 d左右,可达到80%以上的不定芽再生率。而经过农杆菌侵染后,以MS+2 mg/L 6-BA+1 mg/L NAA+1 mg/L KT为共培养基,MS+3 mg/L 6-BA为筛选培养基时,并在50 mg/L卡那浓度筛选下,仍可获得13.3%的抗性芽再生率。不定芽切下放置于生根培养基(1/2MS+3 mg/L IBA+1 g/L 活性炭)中获得86%左右的生根率。通过对影响不定芽和抗性芽再生频率的各种因素进行研究,最终建立了一套有效的金柑实生苗节间茎段离体再生体系。%Kumquat is conducive to the studies of flower and fruit traits,and related gene expression in the flower and fruit using genetic transformation since juvenile period is short and may blossom many times per year,but the rate of seedling regeneration in kumquat still needs to be improved.This study used internode stem segments of‘Yangshuo’ Kumquat(Fortunella spp.Swingle) in Guangxi as the materials.When using the seed-lings cultured in darkness for 25 days and light for 10 days as explants and MS+3 mg/L 6-BA as regeneration culture medium to grow about 50 days, the adventitious bud regeneration rate could exceed 80%. And after agrobacterium infection,using co-cultivation medium( MS + 2 mg/L 6-BA + 1 mg/L NAA + 1 mg/L KT) and selection medium(MS + 3 mg/L 6-BA+ 50 mg/L Kanamycin) as culture conditions,13.3% resistant shoot regeneration rate was obtained.The buds were cut down from the explants and placed in the rooting medi-um (1/2 MS+3 mg/L IBA+1 g/L activated carbon) 86%of the rooting rate was obtained.Finally,the research established an effective regeneration system by studying the various factors affecting regeneration frequency of adventitious shoots and resistant shoots.
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