甜樱桃砧木品种‘吉塞拉6号’的离体培养与快速繁殖

摘要

The stem segments were used as explants,and rapid propagation of gean dwarf stock variety‘Gisela 6’were studied.The results showed that the explants were cultured with the modified MS +1 mg?L ZT +0.5 mg?L BA +0.2 mg?L NAA +0.5 g?L CH,and 30 days later the germination rate of axillary buds was 81.3%;The suitable medium for the bud multiplication was the modified MS +0.5 mg?L BA +0.5 mg?L KT +0.1 mg?L NAA +0.01 mg?L TDZ,and the propagation coefficient reached 5.8;The suitable medium of inducing the test-tube plantlets to root was 1 ?2 MS +1.0 mg?L IBA +0.2 mg?L NAA,and the rooting percentage was up to 98.3%;For the test-tube plantlets to be transplanted the suitable volumetric ratio of the substrate peat +coconut husk +perlite was 1∶1∶1(v?v?v),and the survival rate of test-tube plantlets was over 98%.%以植株茎段为外植体，进行了甜樱桃矮化砧木品种‘吉塞拉6号’离体快速繁殖技术研究。结果显示：外植体培养于改良 MS ＋1 mg？L ZT ＋0．5 mg？L BA ＋0．2 mg？L NAA ＋0．5 g？L CH，30 d 后腋芽萌发率达81.3％；丛芽增殖的适宜培养基是：改良 MS ＋0．5 mg？L BA ＋0．5 mg？L KT ＋0．1 mg？L NAA ＋0．01 mg？L TDZ，增殖系数达5．8；1？2 MS ＋1．0 mg？L IBA ＋0．2 mg？L NAA 是诱导甜樱桃试管苗生根的适宜培养基，生根率达98．3％；适宜试管苗移栽的基质配比为泥炭∶椰糠∶珍珠岩＝1∶1∶1（v∶v∶v），试管苗移栽成活率达98％以上。