The reciprocal cross between glyphosate-resistant plus insect-resistant cotton material and disease-resistant cot-ton material was carried out, and two reciprocal cross combinations were obtained.The agronomic characters, yield characters, fiber quality traits, resistance and other characters of two combinations, their parents, and control variety Ganmianza 1 ( mainly-culti-vated local hybrid cotton variety) were compared, and the three-resistant ( insect-resistant, disease-resistant, and glyphosate-resistant) characters of two hybrid combinations as well as their heterosis performance were studied.The hybrids showed a positive competitive advantage in flowing date, boll opening date, boll-setting schedule, number of fruit branches, single-boll weight, seed cotton yield, lint yield, rate of flowers before frost, and fiber quality, and revealed a negative competitive advantage in knot height of first fruit branch, intersection angle between fruit branch and stem, and growth and developmental duration.The independ-ent resistances of two parents could be relatively steadily inherited to the next generation.The two reciprocal cross combinations all had three-resistant characters simultaneously.%以抗草甘膦抗虫棉材料、抗病棉材料为亲本，进行正反交配组，比较了正反交组合、两个亲本及当地主栽杂交种（对照）的农艺性状、产量性状、纤维品质、抗性等，研究了其抗虫、抗病、抗草甘膦的性状以及杂种优势表现。结果表明：开花期、吐絮期、结铃进度、果枝数、单铃重、籽棉产量、皮棉产量、霜前花率、纤维品质表现为正向竞争优势；果枝始节节位高度、果枝夹角、生育期表现为负向竞争优势；两个亲本的独立抗性能够比较稳定地遗传；正反交组合都同时具有抗虫、抗病、抗草甘膦的三抗性状。
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