The feasibility of distinguishing male and female silkworm chrysalis in cocoon by NIR with fiber optics was discussed. Altogether 187 samples were taken from which 100 samples were used for calibration and others for prediction. "1"and"0"were designed as male and female silkworm chrysalis,respectively’,for regression. Statistical analysis results showed that MR was 0. 930 and SEC 0. 189. The prediction results of 87 samples indicated that R was 0. 937 and SEP 0. 175 respectively. The accuracy of distinguishing sex of silkworm chrysalis in cocoon reached 98. 9%. At the same time,it is possible to discriminate the dead silkworm pupa in cocoon immediately through the spectrum.
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