盐胁迫与非胁迫条件下控制番茄苗期重要植物学性状的QTL对比研究

摘要

利用来源于栽培番茄9706和野生契斯曼尼番茄LA0317杂交后的130个BC2S4株系为群体,采用营养液栽培法,在盐胁迫与非胁迫环境下分别对株高、茎粗、根鲜质量、根干质量、地上鲜质量、地上干质量以及耐盐级数进行了QTL分析.盐胁迫环境中检出13个QTL:株高2个；茎粗2个；根鲜质量2个；根干质量2个；地上鲜质量3个；地上干质量2个.非盐胁迫环境中检出32个QTL:株高6个；茎粗2个；根鲜质量6个；根干质量6个；地上鲜质量6个；地上干质量6个.另外,在盐胁迫条件下利用耐盐级数定位到2个耐盐性QTL,分别位于第3,6染色体上.通过盐胁迫与非胁迫下的QTL对比研究发现,第2,3,8染色体上的QTL仅在盐胁迫条件下能检测到,第9,12染色体上的QTL仅在非盐胁迫条件下能检测到,不同性状间的QTL存在共定位现象.同时,研究获得了5个表型和QTL分析均极耐盐的育种材料,可用于番茄耐盐新品种选育.%An advanced backcross population of 130 BC2S4 individuals was developed derived from the cross Solarium lycopersicum 9706 × Solarium cheesmanii LA0317. Six important phytological traits were investigated under salt stress and nonstress conditions, and salt survival value was also evaluated. Using MQM mapping method, a total of 45 QTL for phytological traits of tomato were detected. 2 QTL for salt tolerance were mapped to chromosome 3 and 6. Through comparative QTL analysis, it was found that QTL on chromosome 2, 3 and 8 could only be detected under salt treatment, while QTL on chromosome 9 and 12 could only be detected under non-salt condition. QTL co-location among different traits was common in this study. Moreover, 5 families of BC2S4 were found in this paper to be salt tolerant, thus these lines could be used as good breeding germplasm.