水稻编码 SAND 结构域基因的序列与功能初步分析

摘要

为研究水稻 SAND 结构域蛋白的序列和功能，利用 Blast 在水稻基因组中搜索编码 SAND 结构域的基因，然后构建增强表达载体并通过遗传转化研究基因功能。结果表明，水稻基因 LOC＿Os01g57240（命名为 OsULT1）编码的氨基酸序列含有 SAND 结构域和 ULT B-box 共有序列，与其他植物 ULT 氨基酸序列相似性为49．3％～92．3％，具有较好的保守性；35S ：OsULT1转基因株系部分颖花发育异常，出现内稃发育滞缓或退化、浆片过度发育或数目变多、雄蕊数目3～7枚、雌蕊2枚的现象；颖花内产生多余的小花，表明 OsULT1对水稻花分生组织正常分化具有重要的调控作用。%To study the sequence and function of rice SAND domain protein,the gene encoding SAND was identi-fied by Blast against the rice genome,and then over-expression vector was constructed to study its gene function by ge-netic transformation.The results found that the rice genome contains a gene LOC_Os01g57240(named as OsULT1),which encoded amino acid sequence containing SAND domain and ULT B-box consensus sequence.The amino acid sequence similarity between OsULT1 and ULTs of other plants varied from 49.3% to 92.3%,they were highly conservative.The 35S ::OsULT1 transgenic lines generated some mutant spikilets,which included under-developed or degenerated pa-leas,over-developed locidules,the increased number of locidules,the variant number of stamen,two pistils,or extra floret.The results indicates OsULT1 should play important roles in regulation of floral meristem identity in rice.