β-巯基乙醇和发情羊血清对牛颗粒细胞体外培养的影响

摘要

This experiment studied the bovine culumus cells in vitro culture in order to satisfy the needs for culumus cells,enhance the ability of ovocyte maturine and embryo development in vitro and investigate the morphological characteristics and functions of culumus cells. The growth of culums cells was observed after adding fi-mercaptoethanol (BME) in different concentrations, 12. 5,25,50, 100,200 fzmol/L to the TCM199 medium (Ph7. 4) ; meanwhile, the numbers of culums cells were observed after adding 10% of different period of estrus sheep serum(ESS) to the basic TCM199 medium. The vitality increased as the rising of BME concentration. There was a turning point in 25 fzmol/L, then the vitality decreased; The number of culumus cells was maximum at the group of serum collected at the first day of sheep oestrum(ESS Id). BME can increase the vitality of culumus cells and the best concentration was 25 jumol/L. The serum collected at the first day of sheep oestrum (ESS Id) was the most suitable for culumus cells in vitro culture system.%为满足实验室卵母细胞体外成熟共培养和胚胎共培养的需要以及进行卵巢颗粒细胞生物学功能等研究的需要,进行牛卵巢颗粒细胞的体外培养研究.在基础培养液TCM199中分别添加12.5、25、50、100、200 μmol/L的β-巯基乙醇(BME),观察颗粒细胞生长状况；在基础培养液中分别添加体积分数为10％的发情山羊第1天血清、体积分数为10％的发情山羊第11天血清和体积分数为10％的胎牛血清,观察颗粒细胞生长和增殖情况.体外培养颗粒细胞的活细胞率随着添加BME浓度的增大而增加,在25 μmol/L时达到最高,此后随浓度的增大而降低；发情山羊第1天的血清组中,颗粒细胞生长和增殖情况最好.BME可以提高颗粒细胞体外培养的活率,最佳添加浓度为25 μmol/L；发情山羊第1天的血清可有效促进颗粒细胞体外培养生长和增殖.