通过对传统玻片标本制作方法的比较,研究牛、羊消化道线虫虫卵永久性玻片标本制作的最佳方法.采用琼脂糖粉分别配制10和15g/L的水溶液作为介质.载玻片上加入少许玻璃碎渣,用毛细吸管吸取一小滴虫卵液(约10个虫卵)于玻璃渣上,再吸取一滴介质于虫卵上,盖上小盖玻片(10 mm×10 mm),待介质凝固后,滴树胶并加盖玻片(18 mm ×18 mm).制作的玻片中虫卵经长时间保存后,能保持原来的形态及色泽,清晰可见,易于辨认.琼脂糖为介质的水溶性虫卵玻片的制作解决了传统方法制作玻片标本易出现气泡、空隙、发霉及虫卵变形等的问题,是虫卵玻片标本理想的制作方法,可供临床和教学实习使用.%In order to optimize the method for preparing permanent slide specimen, agarose solution (10 and 15 g/L) was used as a medium to prepare the permanent slide specimen of gastrointestinal nematode eggs of cattle and sheep. Firstly, we put a few of glass residue onto a glass slide with tweezers, and draw a little drop of eggs on the blizzards with a capillary suction (about 10 eggs). Following, the eggs should be shield by another drop of straw medium (warm agarose medium in a small flask in water bathe), then they must be covered with a cover slip (10 mm× 10 mm) immediately. After the medium shielding the eggs was solidified, the slide specimen should be fixed with resin and another cover slip (18 mm× 18 mm). By the experiment, we found that the eggs preserved by the method not only maintained a good shape and color but also were clearly visible and legible. As a result, we believed that it is a kind of suitable method to preserve nematode eggs. The water-soluble agarose medium effectively solved the problems of the traditional methods, which are easy to cause bubbles, voids and mildew, egg deformation, inconvenience observation and preservation issues, and our work here provided an alternative method to facilitate clinical and teaching practice.
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