Stem-cell based bladder tissue engineering potential role of bone marrow mesenchymal stem cells (BM-MSC) as smooth muscle cell (SMC) progenitors.

摘要

Objective. This thesis focused on the potential of BM-MSC for SMC differentiation.;Results. BM-MSC differentiated to bone and fat and expressed characteristic surface markers. In stem cell medium BM-MSC expressed all three SMC markers. Low serum significantly reduced SMC differentiation and proliferation. TGF-beta1 and PDGF-BB enhanced SMC differentiation in 10% and 20% serum. Sca-1 positive cells showed decreased SMC differentiation potential.;Conclusion. BM-MSCs undergo spontaneous reversible SMC differentiation in culture. These observations suggest that SMC differentiation requires the coordinated promotion between serum factors and exogenous growth factors.;Methods. SMC differentiation of characterized BM-MSC was assessed with SMC markers: alpha-smooth muscle actin (alpha-SMA), calponin, and smooth muscle myosin heavy chain (MHC) in stem cell media, in low serum conditions, and after stimulation with TGF-beta1 and PDGF-BB. I compared sorted Sca-1 positive cells from BM-MSC cultures and evaluated SMC differentiation by immunofluorescence and western blot. Bladder SMCs were used as controls.