Advancement towards a Biorefinery Platform Using the Cyanobacterium Synechococcus sp. Strain PCC 7002.

摘要

Cyanobacteria are prokaryotic oxygen photoautotrophs that have high areal productivity and can thrive in diverse ecosystems. These features have made them attractive biological catalysts for the sustainable production of specialty and commodity chemicals. This thesis examines the use of the exceptionally tolerant cyanobacterium, Synechococcus sp. strain PCC 7002, for the ability to use a municipal wastewater stream as a nutrient source under several conditions. We explore the capacity of Synechococcus sp. strain PCC 7002 for the production for proof of concept molecules, L-lactate and L-lysine. We discuss metabolic engineering design principles that enable high productivities and carbon partitioning, with an emphasis on leveraging the coordination between carbon and nitrogen metabolism.