Kinetics of phenol degradation by Pseudomonas putida Al isolated from a tropical soil.

摘要

Various methods have been proposed to control synthetic solvents from wastewater. The principal goal is to reduce or to eliminate organic contaminants (minimization). Biological treatments of wastewaters containing low concentrations of pollutants is one of the cheapest and most effective technologies available to industries.;This work deals with the biotreatment of water containing phenol or 1,1,1-trichloroethane (TCA) using isolated microorganisms of heterogeneous cultures from previously contaminated sites. The specific objectives for this research were to use these isolated microorganisms to determine their tolerance to an aqueous phase containing the toxic chemicals and the tolerable levels, and to determine the growth kinetics and the kinetics of degradation.;Microorganisms collected from different sites that could grow in media containing phenol in concentrations varying from 10 to 50 mg/L and in media containing TCA in concentrations varying from 10 to 30 mg/L of TCA were isolated. Pseudomonas putida A1 and Salmonella sp., were isolated from material collected at the Guayanilla Petrochemical Complex. Experimental trials with artificial phenol solutions at five initial concentrations levels were carried out and its concentration variation quantified with respect of time. Two general behaviors in the kinetics of compound degradation were obtained; at low concentrations levels the behavior was of the first order degradation kinetic reaction and at levels over concentration of 30 mg/L the behavior was of zero order.;Microbiological growth was quantified to establish its relation to the substrate concentration and time. Finally, Monod type behavior kinetic parameters were determined for the aqueous phase phenol degradation with Pseudomonas putida. (Abstract shortened by UMI.).