The subject of my thesis is the investigation of molecularly imprinted polymers (MIP), in particular with respect to their analytical applications.;MIPs are selective sorbents. Selective adsorption is a practically useful property, e.g., in environmental technology, in chemical industry and in analytical chemistry. This explains the wideranging interest for MIPs.;The adsorption isotherms of MIPs are usually nonlinear even down to very low concentrations. The far reaching consequences of this nonlinearity have not been fully recognized yet. Some of the important consequences are being described in my thesis. I have created and verified a novel experimental method for detecting the nonlinear chromatographic behavior of MIP stationary phases in the concentration range of their intended application. I have proved the nonlinear chromatographic behavior of a phenytoin imprinted MIP made with methacrylic amide functional monomer in the pores of a modified silica stationary phase.;I have proved both by experiments and by computer modeling that the characterization of MIPs by chromatographic experimental parameters like k, alpha and the imprinting factor (IF) is not appropriate. I have discovered that these parameters, which are all based on measuring the position of the peak maximum, show an unexpected dependence on apparently irrelevant experimental data like the length or inner diameter of the chromatographic column.;I have shown experimentally and by computer modeling that competition between coinjected analytes is much less discernible in MIP HPLC than in non-transient techniques like batch competitive binding assays or sensors.
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