Multimodal and Interactive Micro-Biomanipulation: From Sensors Development, Mind-controlled Interface to Network-enabled System Integration.

摘要

Micro-biomanipulation is an advanced technology that aims at the development and application of advanced sensing/imaging and automation technologies to improve processes involving the manipulation (injection, alignment, separation, patch clamp, etc.) and in-vitro mechanical or electronic characterization of biological entities such as individual cells, early embryos, and tissues. In this thesis, we describe a cost-effective, highly efficient, multi-degree-of-freedom, and multi-modal micro-biomanipulation research platform that is in a symbiotic relationship with human operators to improve processes of micro/nano biomechanical characterization and its automation level. As a first step, several important sensors and interfaces for our multi-modal and interactive micro-biomanipulation system are developed, custom designed, and tested, including a position sensitive detector (PSD) based micro/nano position sensing system, a non-invasive Electroencephalography (EEG) interactive interface, and hybrid piezoresistive (HP) based highly sensitive 1-D and 3-D microforce sensors with self-decoupling functions. Beyond that, relying on these developed sensors and interface, a network-enabled system architecture is established and three system applications consisting of (1) musical tuning enhanced micro palpation of biological entities; (2) brain-driven micro-biomanipulation; and (3) a portable radial artery pulse sensing system are successfully conducted and demonstrated. Extensive simulation and experimental results validate that our work is a major step toward a multi-modal and interactive micro-biomanipulation system for varied biomedical applications.