A novel groundwater and soil remediation technique using peroxidases.

摘要

The ability of horseradish peroxidase (HRP) and lignin peroxidase (Lip) to remediate aqueous and solid media impacted with petroleum hydrocarbons was studied. The enzymes were introduced to the impacted media in soluble and immobilized form.;175 mg of gasoline (aqueous media) was remediated over 6 days using 2 mg of enzymes immobilized onto 5.375 g of activated carbon and 42 mg of gasoline (solid media) was remediated over 48 hours using 1 mg of enzymes immobilized onto 2 g of activated carbon.;Combining activated carbon with enzymes enhanced removal compared to using soluble enzymes alone; the carbon may have preferentially absorbed the pollutant, bringing it into close proximity with the enzymes that facilitate conversion.;The best contaminant degradation (99.1% and 96.1%, respectively) was achieved using immobilized enzymes for both the impacted groundwater and soil media samples. Groundwater treatment via a PBR achieved the highest contaminant reduction of over 99.9%.