Novel Injectable Hydrogel Coated with Helical Rosette Nanotubes for Bone Tissue Engineering

摘要

Bone tissue engineering aims to induce new functional bone regeneration by the synergistic combination of biomaterials, cells, and factor therapy. Smart biomaterials like injectable hydrogels will empower clinicians to treat and regenerate tissues in ways that are previously tough and impossible. They have good biocompatibility, excellent permeability due to their porous structure, and other attractive features including minimal invasion and limitless supply. Our hydrogel composed of 15% p(NiPAAm-c0-GMA) and 10%PAMMAM, chemical gelation led by pendant epoxy group and secondary amine group was proved. Helical Rosette Nanotubes (HRNs) self-assemble in physiological environments. Coated on the surface, K1 HRNs were used in this thesis for bone tissue engineering in terms of improve the adhesion and proliferation of osteoblast cells. They were proved to provide better mechanical properties and cytocompatibility when dissolved in hydrogel solution and with a concentration of 2.5mg/mL among 1mg/mL and 5mg/mL . Our study aim to focus on more concentration comparison and the behavior when HRNs are coated on the surface of hydrogel materials. Results of this study showed the first time a concentration range of an optimal condition when coating HRNs, which is 1.75-2.5mg/mL . Significant difference between control and 1mg/mL coating, and also when increased from 1mg/mL to 1.75 mg/mL especially for longer osteoblast cell culture time period which demonstrated that the hybrid scaffolds composed of 15% p(NiPAAm-co-GMA) and 10% PAMAM and 1.75-2.5mg/mL HRNs have a strong potential in bone tissue engineering.