声明
ABBREVIATIONS
Chapter Ⅰ An Overview of the Central Nerve System and Oligodendrocytes
1. Introduction
1.1A Central Dogma in Neurobiology
1.2 The Role of Oligodendrocytes in Nerves System
2. The Aim of This Thesis
Chapter Ⅱ Comparative Study on the Differentiation and Induction of NSCs into Oligodendrocytes by Traditional and New Methods
1. Introduction
2. Materials and Methods
2.1Animals
2.2 Tools and Equipment
2.3 Medium and Reagents
2.4 Mouse NSCs Isolation and Culture in Vitro.
2.5 ImmunocytochemistryAnalysis
2.6 Induce NSCs to Oligodendrocytes on Colony Level
2.7 RT-PCR assay
3. Results
3.1 Identification of NSCs
3.2 Induce NSCs to Oligodendrocytes
4. Discussion
5. Conclusion
Chapter Ⅲ Study of High Efficiently Induction of NSCs into OLs under Defined Condition
1. Introduction
2. Materials and Methods
2.1 Medium, Reagents and Tools
2.2 Induce NSCs into Oligodendrocytes by KSR with Growth Factors
2.3 Treating Culture Dish with Laminin and Poly-L-Lysine
2.4 RT-PCRAssay
2.5 ImmunocytochemistryAnalysis
3. Results
3.1 Induce NSCs to Oligodendrocytes by KSR with Growth Factors
3.2 Culture Neural Stem Cells on Laminin and Poly-L-Lysine Co-coated Surface
3.3 Induce NSCs towards Oligodendrocytes on Laminin and Poly-L-Lysine Co-coated Surface
3.4 Immunocytochemistry Analysis of OLs Induced on Laminin and Poly-L-Lysine Co-coated Surface
4. Discussion
5. Conclusion and Prospect
参考文献
致谢
Participated in International Conferences
List of Publications
内蒙古大学;
