声明
提要
Preface
Abbreviations
1 Literature review
1.1 AIRE as an important regulator for T cell development
1.1.1 Mechanism of tolerance at Thymus
1.1.2 Molecular features of AIRE functioning
1.2 AIRE Ahead of Auto antigens
1.3 Anti-tumor immunity of AIRE
1.4 TSA independent functions of AIRE
1.5 Activity of AIRE in GVHD
1.6 Extra thymic expression of AIRE and its role in peripheral tolerance
1.6.1 AIREs Tissue and Cellular Distributions
1.6.2 AIRE's function in Peripheral Immune Tolerance
1.7 Immune Response to Candida albicans
1.7.1 Antimicrobial proteins against C. albicans
1.8 Response of Macrophage to Candida albicans components
1.8.1 Recognition of β-1,6-glucans of C. albicans by Dectin-1 of Macrophage
1.8.2 Macrophages response to cell wall chitin
1.8.3 Mannoprotiens interaction with macrophage
1.9 Proinflammatory macrophages M1 and anti-inflammatory M2 and their response to Candida albicans
1.10 Secretion of cytokines in the response of Candida albicans
1.11 Candida albicans infections as a common consequence of APECED patients
2 Study design
3. Material and method
3.1.1 Mice
3.1.2 Candida albicans strain
3.1.3 Main reagents
3.1.4 Main equipment
3.1.5 Reagents preparation
3.2 Methods
3.2.1 Isolation and polarization of mice peritoneal macrophage
3.2.2 Flow cytometry
3.2.3 Establishment of systemic candidiasis model
3.2.4 Macrophage and candida albicans co-culturing
3.2.5 RNA isolation and RT-qPCR analysis
3.2.6 Statistical Analysis
4 Results
4.1 Effects of AIRE deficiency on macrophage response to Candida
4.1.1 The culture of Candida albicans
4.1.2 The macrophage isolation and identification from mice peritoneal
4.1.3 The stimulation of Candida albicans to macrophage
4.1.4 Effects of AIRE on expression of M1 associated molecules in response to Candida albicans
4.1.5 Effects of AIRE on PRR expression of macrophages in response to
4.2 Effects of AIRE deficiency on the macrophage response to Candida albicans in vivo
4.2.1 Observation of the general status of Candida albicans infected mice
4.2.2 The effect of AIRE deficiency on the histological pathology in mice
4.2.3 The isolation and identification of peritoneal cavity macrophage from mice with Candida albicans infection
4.2.4 The effect of AIRE deficiency on M1 related molecules expression in macrophage from Candida albicans infected mice
4.2.5 The effect of AIRE deficiency on the survival of mice with Candida
5 Discussion
6 Conclusion
参考文献
Short introduction of the writer and attainment throughout research at school
致谢
吉林大学;
