声明
Acronyms and Abbreviations
Chapter 1 Introduction
1.1 Background
1.1.1 Historicity of disinfection
1.1.2 WHO’s role in drinking water safety
1.1.3US EPA Standards for DBPs
1.1.4US EPA Rules History for DBPs
1.1.6 Chloroacetic acid
1.1.7 2,6-dichloro-1,4-benzoquinone
1.1.8 SH-SY5Y cell line
1.2 Rationale
1.3 Objective
Chapter 2 Materials and Methodology
2.1 Materials
2.2 List of Instruments
2.3.1 1× PBS solution
2.3.2 1×Trypsin
2.3.3 Methyl thiazolyl tetrazolium (MTT) solution
2.3.4 Dulbecco′s Modified Eagle′s Mediumculture media
2.3.5 Retinoic acid solution
2.3.6 2,6-DCBQ stock solution
2.3.7 CAA stock solution
2.4 Work flow chart
2.5 Aseptic working environment
2.6 Cell culture
2.6.1 Counting of cells
2.6.2 Subculture and differentiation of cells
2.7 SH-SY5Y exposure to CAA and 2,6-DCBQ
2.8 Cell Viability
2.9Neurite Outgrowth
2.10 Immunofluorescence microscopy for β-tubulin
2.11 Ratio of different cell populations
2.12 Flow cytometry of cell cycle
2.13 Cells growth rate and doubling time
2.14 Morphological analysis of undifferentiated and differentiating SH-SY5Y cells
2.15Statistical analysis
Chapter 3 Results
3.1 The toxic effects of CAA and 2,6-DCBQ on the undifferentiated SH-SY5Y cells
3.1.1 The cell growth curves of the undifferentiated SH-SY5Y cells
3.1.2 The effects of CAA and 2,6-DCBQ on the proliferation of the undifferentiated SH-SY5Y cells
3.1.3 The effects of CAA and 2,6-DCBQ on themorphology of undifferentiated SH-SY5Y cells
3.1.4 The effects of CAA and 2,6-DCBQ on the cell cycle of the undifferentiated SH-SY5Y cells
3.2 The toxic effects of CAA and 2,6-DCBQ on the neural differentiating SH-SY5Y cells
3.2.1 The characterization of the neural differentiating SH-SY5Y cells
3.2.2 The cell growth curves of the neural differentiating SH-SY5Y cells
3.2.3 The effects of CAA and 2,6-DCBQ on the proliferation of the neural differentiating SH-SY5Y cells
3.2.4 The effects of CAA and 2,6-DCBQ on the morphology of differentiating SH-SY5Ycells
3.2.5 CAA and 2,6-DCBQ affect the neural differentiating of SH-SY5Y cells
3.2.6 CAA and 2,6-DCBQ affect the cell cycle of neural differentiating SH-SY5Y cells
Chapter 4 Discussion and Conclusion
4.1 Discussion
4.1.1 Toxic effects of CAA and 2,6-DCBQ on the undifferentiated SH-SY5Y cells
4.1.2 Toxic effects of CAA and 2,6-DCBQ on neural differentiating SH-SY5Y cells
4.2 Conclusion
参考文献
致谢
吉林大学;
