声明
Abbreviations
Chapter Ⅰ Introduction
1.1 Effects of drought stress on plants
1.1.1 Crop growth and yield
1.1.2 Water relations
1.1.3 Nutrient relations
1.1.4 Photosynthesis
1.1.5 Assimilate partitioning
1.1.6 Respiration
1.1.7 Oxidative damage
1.2 Drought resistant mechanisms
1.2.1 Morphological mechanisms
1.2.2 Physiological mechanisms
1.2.3 Molecular mechanisms
1.3 Drought-hardening
Chapter Ⅱ Transcriptome Profiling, Biochemical and Physiological Analyses Provide New Insights towards Drought Tolerance in Tobacco
2.1 Introduction
2.2 Materials and Methods
2.2.1 Plant materials and drought stress treatments
2.2.2 Morpho-physiological parameter measurement in response to drought stress
2.2.3 Determination of biochemical parameters in response to drought stress
2.2.4 Histological studies
2.2.5 RNA extraction, cDNA library construction, and transcriptome sequencing
1.1.1 2.2.6 Validation of RNA sequencing data through quantitative real-time PCR (qRT-PCR) analysis
2.2.7 Statistical analysis
2.3 Results
2.3.1 Effects of drought stress on plant growth, chlorophyll content, and leaf water potential of the three tobacco varieties
2.3.2 Chlorophyll fluorescence and multicolor fluorescence parameters in response to drought stress
2.3.3 Oxidative damage (MDA and H2O2 contents) in response to drought stress
2.3.4 Proline and soluble sugar contents in response to drought stress
2.3.5 Antioxidant enzyme activity in response to drought stress
2.3.6 Non-enzymatic antioxidant components in response to drought stress
2.3.7 Leaf anatomy in response to drought stress
2.3.8 Summary statistics of reads in the leaves transcriptome of three tobacco varieties in response to drought stress
2.3.9 Gene ontology (GO) and KEGG enrichment analyses of tobacco transcriptomes
2.3.10 Plant hormone signal transduction in response to drought stress
2.3.11 Starch and sucrose metabolism in response to drought stress
2.3.12 Proline metabolism in response to drought stress
2.4 Discussion
2.4.1 Growth, chlorophyll content, and leaf water potential of the three tobacco varieties in response to drought stress
2.4.2 Chlorophyll fluorescence and multicolor analyses under drought stress
2.4.3 Oxidative damage caused by drought stress in the three tobacco varieties
2.4.4 Enzymatic antioxidant defense system alleviates the drought stress response in the three tobacco varieties
2.4.5 Non-enzymatic antioxidant defense system involved in mitigating the adverse effect of drought stress
2.4.6 Proline and soluble sugars improve drought tolerance in the three tobacco varieties
2.4.7 Leaf anatomical modification in the three tobacco varieties under drought stress
2.4.8 Analysis of plant hormone signal transduction in response to drought stress
2.4.9 Analysis of starch and sucrose metabolism in response to drought stress
2.4.10 Analysis of proline metabolism in response to drought stress
Chapter Ⅲ Drought-Hardening Improves Drought Tolerance in Tobacco at Physiological, Biochemical, and Molecular Levels
3.1 Introduction
3.2 Materials and Methods
3.2.1 Plant materials, growth conditions, and drought-hardening
3.2.2 Expression analysis of the selected drought-responsive genes through qRT-PCR
3.2.3 Statistical analysis
3.3 Results
3.3.1 Effect of drought-hardening on growth, leaf water potential, chlorophyll fluorescence, and multicolor fluorescence of two tobacco varieties in response to drought stress
3.3.2 The evaluation of drought-hardening on the oxidative damage (malondialdehyde (MDA), hydrogen peroxide (H2O2)) in response to drought stress
3.3.3 The assessment of drought-hardening on enzymatic antioxidant defense system in response to drought stress
3.3.4 The effect of drought-hardening on non-enzymatic antioxidant defense system in response to drought stress
3.3.5 The influence of drought-hardening on proline and soluble sugar contents in response to drought stress
3.3.6 Drought-hardening effect on leaf anatomical modifications in response to drought stress
3.3.7 Drought-hardening activated the expression of potential drought responsive genes during drought stress
3.4 Discussion
3.4.1 Manifestations in chlorophyll fluorescence and multicolor fluorescence parameters caused by drought-hardening in response to drought stress
3.4.2 Drought-hardening alleviates the oxidative damage during drought stress
3.4.3 Drought-hardening improve drought tolerance via enhanced enzymatic antioxidant defense system
3.4.4 Positive impact of drought-hardening on non-enzymatic antioxidant defense system results in drought tolerance
3.4.5 Drought-hardening improve proline and soluble sugars involve in mitigating the adverse effects of drought stress
3.4.6 Leaf anatomical modifications in the two tobacco varieties of drought-hardened and non-drought-hardened seedlings under drought stress
3.4.7 Drought-hardening improve the expression of potential drought responsive genes during drought stress
Chapter Ⅳ Conclusion
参考文献
Appendix A
致谢
Author Biography
中国农业科学院;
