Neuronal Type Specific Fluorescence Labeling in C.elegans

摘要

Caenorhabditis elegans (C.elegans) are studied due to its useful systems.It offers various neurobiological processes at the molecular and cellular level cellular for studying.C.elegans has a very simple nervous system (302 neurons).It explores its environment by interrupting its forward movement with occasional turns and reversals turns.Its reversals turns occur at stable frequencies but in irregular intervals,producing probabilistic exploratory behaviors.In this thesis, we discuss how the neurons circuit controls are used for seeking food behaviors in C.elegans.
　　By stimulating long reversals and omega turns upon removal from food, AWC and ASK play a major role in the local search state.npr-9 is responsible of search/pivoting.It displays an increased local search/pivoting that impairs their ability to travel long distances and roam on for food.Role head motor neurons were manipulated and tested.The frequency of short reversals increased when RIM was killed.On the other hand when both RIM and AVA neurons were killed,the effect of eliminating RIM was suppressed.RIM makes synapses and gap junctions onto the command interneurons,which mean that it may inhibit short reversals through these connections.SMB motor neurons also make synapses onto the command neurons,and it inhibits short reversals.Neither RMD nor SMD synapse onto the command neurons directly.Killing either cell increases the frequency of reversals. Omega turns were stimulated by SMD and RIV motor neurons.Killing SMD and RIV neurons leads to a decrease in the frequency of omega turns.
　　RMG inter/motor neuron is a hub of a gap-junction network connecting seven classes of neurons.RMG neurons stimulate aggregation-related behaviors in npr-1 mutants, and indicate that NPR-1 inhibits RMG activity in solitary strains.When RMG was killed,it resulted in the elimination of aggregation,bordering and rapid movement.AVE Command interneurons along with AVA, AVD and A-type motor neurons drive backward movement of the animal.

目录

封面

声明

英文摘要

目录

1 Introduction

1.1 The anatomy of the C. elegans nervous systems

1.2 The sensory nervous system of Caenorhabditis elegans

1.3 Sensing the environment

1.4 Ion Channel Families That Sense in the Worm

1.5 Seeking food behaviors

2 Technology

2.1 Plasmid Construct

2.2 C. elegans Microinjection

2.3 Live Imaging of Caenorhabditiselegan

3 Results

4 Conclusion

5 Acknowledgements

参考文献