论文说明:Abbreviations
声明
英文文摘
摘要
Chapter 1 Introduction
Photosynthesis, one of the most important biological reactions on earth
Diversity and evolution of photosynthesis
Pigments
Reaction centers
Light-harvesting complexes
Morphology of photosynthetic membranes
Photosynthesis in cyanobacteria and red algae
Individual protein complexes of the photosynthetic apparatus
Molecular architecture of thylakoid membrane
Phycobilisome arrangement on the thylakoid surface
Arrangements of photosystems
Association between phycobilisomes and photosystems
State transition
References
Chapter 2 One-step chromatography method for efficient separation and purification of R-PE from Polysiphonia urceolata
Introduction
Results
The separation of R-PE
The identification to purification of R-PE
The analysis to each separation process
R-PE stability to pH variations
Discussion
Materials and methods
Organisms
Chemicals
Phycoerythrin purification
Determination of purification
R-PE stability to pH variations
References
Chapter 3 Probing protein folding interactions by investigations on active conformation and function of R-phycoerythrin
Introduction
Results
Absorption spectra
CD spectra
Fluorescence spectra of R-PE as a function of pH
Refolding experiment
Discussion
R-PE hexamer assembly and flexible conformation
Aromatic residues in R-PE play essential roles in energy transfer
γsubunit in R-PE
Materials and methods
Sample preparation
Spectral measurement
Structural analysis
References
Chapter 4 Structure and organization of phycobilisomes on membranes of the red alga Porphyridium cruentum with transmission electron microscopy
Introduction
Results
Spectral properties of isolated PBsomes
PBsome components
Electron microscopy
Discussion
Materials and methods
Isolation of isolated PBsomes and PBsome-thylakoid membranes
Spectral analysis
SDS analysis
Electron microscopy
References
Chapter 5 Probing the native architecture and photoacclimation of thylakoid membranes in red algae with atomic force microscopy
Introduction
Results
AFM topography of isolated PBsomes
Native architecture of PBsome-thylakoid membrane
Discussion
Native architecture and photoacclimation of photosynthetic membrane in red algae
Packing of PBsomes on the crowded thylakoid membrane
Configurational heterogeneity of thylakoid membrane
Interactions between PBsomes and PSs
Materials and methods
Separation of intact PBsomes and thylakoid membrane
Spectral analysis
Atomic force microscopy
Transmission electron microscopy
References
Chapter 6 Unrevealing the dynamics of phycobilisomes on the thylakoid membrane in red algae using fluorescence recovery after photobleaching
Introduction
Results
Fluorescence recovery of PBsomes
Fluorescence properties of PE emission during photobleaching
Discussion
Materials and methods
Transmission electron microscopy
FRAP measurements
References
Chapter 7 Light-induced energetic decoupling as a mechanism for phycobilisome-related energy dissipation in red algae: a single molecule study
Introduction
Results
Fluorescence photobleaching of single PBsome
Power-dependence of single PBsomes during photobleaching
Green laser induces an energetic deeoupling of the PBsome
Oxygen-dependence of single PBsome during photobleaehing
Fluorescence photobleaching of mutant PBsome
Discussion
Green-light-induced energetic decoupling of PBsome in red algae
The specific association ofγ-subunit-containing PE
The contribution of PBsomes in the photoprotection of red algae
Materials and methods
Sample separation and immobilization
Single-molecule experimental setup
References
Chapter 8 Dimers of light-harvesting complex 2 from Rhodobacter sphaeroides characterized in reconstituted 2D crystals with atomic force microscopy
Introduction
Results
Discussion
Dimeric LH2
Packing lattices
Tilting of LH2
Materials and methods
LH2 purification
Two-dimensional crystallization
Atomic force microscopy
References
Summary
Acknowledgments
Publications