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英文文摘
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CHAPTER 1 GENERAL INTRODUCTION AND PROJECT LAYOUT
CHAPTER 2 REVIEW OF LITERATURE
2.1 PLANT DEFENSES
2.2 NON-EXPRESSOR OF PATHOGENESIS RELATED GENES 1 (NPR1)
2.2.1 NPR1 in SA mediated SAR
2.2.3 The role of NPR1 in induced systemic resistance (ISR) and JA mediated defense against herbivore (HIR)
2.2.4 Plant defense by NPR1
2.3 ALLENE OXIDE SYNTHASE (AOS)
2.3.1 Copy number of the gene
2.3.2 AOS in the JA signaling pathway
2.3.3 Expression of AOS
2.3.4 Plant defense by AOS
CHAPTER 3 EXPRESSION PROFILE OF NPR1 IN RICE
3.1 INTRODUCTION
3.2 MATERIALS AND METHODS
3.2.1 Plant material
3.2.2 Insects.
3.2.3 Plant treatments
3.2.4 Sample processing
3.2.5 Cloning of NPR1 and sequence analysis
3.2.6 Quantitative real-time PCR (qRT-PCR)
3.3 RESULTS
3.3.1 Cloning, identification of NPR1 and its sequence analysis
3.3.2 NPR1 protein nucleotide and amino acid sequence
3.3.3 Phylogenetic tree of NPR1 and its homologues
3.3.4 Relative expression levels of NPR1 in different rice tissues
3.3.5 Influence of signaling molecules on the expression levels of NPR1
3.3.6 Influence of herbivore infestation on expression levels of NPRI
3.4 DISCUSSIONS
3.4.1 Relative expression levels of NPRI in different rice tissues
3.4.2 Influence of signaling molecules on the expression levels of NPR1
3.4.3 Influence of herbivore infestation on expression levels of NPR1
3.5 CONCLUSIONS AND FUTURE PROSPECTS
CHAPTER 4 GENETIC TRANSFORMATION OF NPR1 (NON-EXPRESSOR OF PATHOGENESIS RELATED GENES-1) IN RICE
4.1 INTRODUCTION
4.2 MATERIALS AND METHODS
4.2.1 Cloning of NPR1 gene fragment into pCAMBIA 1301 vector
4.2.2 Transformation to Agrobacterium
4.2.3 Tissue culture/Callus induction
4.2.4 Bacterium activation and co-cultivation
4.2.5 Selection of the transformed calli
4.2.6 GUS assay of the resistant calli
4.2.7 Regeneration of the resistant calli
4.3 RESULTS AND DISCUSSION
4.3.1 Restriction enzyme digestion of DNA fragment
4.3.2 Successful extraction of pCAMBIA 1301
4.3.3 Identification of the recombinant plasmid
4.3.4 Callus induction
4.3.5 Bacterium and Co-cultivation
4.3.6 Selection and plant regeneration
4.3.7 Transformation efficiency
CHAPTER 5 CLONING AND GENETIC TRANSFORMATION OF AOS (ALLENE OXIDE SYNTHASE) IN RICE
5.1 INTRODUCTION
5.2 MATERIALS AND METHODS
5.2.1 Plant material
5.2.2 Isolation of total RNA
5.2.3 Reverse Transcription
5.2.4 Polymerase Chain Reaction
5.2.5 Cloning of AOS gene fragment into pMD19-T vector
5.2.6 Cloning of AOS gene fragment into pCAMBIA 1301 vector
5.2.7 Transformation to Agrobacterium
5.3 RESULTS AND DISCUSSION
5.3.1 Cloning and identification of AOS and its sequence analysis
5.3.2 AOS protein nucleotide and amino acid sequence
5.3.3 Phylogenetic tree of AOS and its homologues in different plant species
5.3.4 Successful extraction of pCAMBIA 1301
5.3.5 Restriction enzyme digestion of the DNA fragment
5.3.6 Identification of the recombinant plasmid
5.3.7 Callus induction
5.3.8 Bacterium and co-cultivation
5.3.9 GUS Histochemical Assay, election and plant regeneration
5.3.10 Transformation efficiency
CHAPTER 6 CONCLUSIONS AND FUTURE PROSPECTS
6.1 CONCLUSIONS
6.2 RECOMMENDATIONS
REFERENCES
APPENDIX
CHAPTER 7 DIFFERENTIAL ATTRACTION OF PARASITOIDS IN RELATION TO SPECIFICITY OF KAIROMONES FROM HERBIVORE AND ITS BY-PRODUCTS
CURRICULUM VITAE
