Study on Molecular Bond-Rupture Events by Quartz Crystal Microbalance

摘要

The bond-rupture scaruung used for biomedical diagnostics is studied with quartzcrystal nucrobalance (QCM) experiments and micro-particle's mechanics modelingcalculation and simulation. The specific and non-specific interactions between amicro-particle and its binding QCM surface can be distinguished by gradually increasingthe amplitude of driving voltage applied to QCM, and morutoring its frequency changes.The research first proposed a 3-D model ofinteraction process ofmicroparticles and QCM,The investigation of the effect of various external factors on QCM's vibration, is essentialfor calculation of bond-rupture force and understanding the interaction mechanism of themicroparticles and QCM. More important, this thesis provided a new one pivot-pointbottom-up vibration mechanical model to calculate the bond-rupture force quantitatively.The selection of the microparticle radius, QCM resonance frequency and the voltageamplitude in the biosensor should be considered carefully. The results will provide validreference data for QCM detecting experiments. The results have evidenced greatsignificance to theoretical research and practical applications of a bond-rupture biosensor,paving a way of extensively spreading in the related areas.