第一个书签之前
声明
致谢
Introduction
Chapter 1:Literature review
1.1. An overview of genetic modification
1.2. Gene-editing nucleases:from meganucleases to CRISPR/Cas systems
1.2.1. Meganucleases
1.2.2. Zinc finger nucleases (ZFNs)
1.2.3. Transcription activator-like effector nucleases (TALENs)
1.2.4. Clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR-associated proteins (Cas)
1.3. A glimpse on CRISPR systems: from observed short repetitions to versatile genome editing tools
1.4. Applications of CRISPR/Cas systems in yeast
1.5. Applications of CRISPR/Cas systems in animals
1.5.1. Sheep
1.5.2. Goats
1.5.3. Cattle
1.5.4. Pigs
1.5.5. Rabbits
1.5.6. Chickens
Chapter 2:Using T7-sgRNAs to evaluate the efficiencies of different target sites in yeast
2.1. Materials and methods
2.1.1. Strains, plasmids, and media
2.1.2. Generation of linear T7-sgRNAs
2.1.3. Yeast transformation
2.2. Results
2.3. Discussion
2.4. Summary
参考文献
Appendices
About the author
Publications
