人源Naive Fab抗体库的构建和抗Met抗体片断的筛选及特征鉴定

摘要

本研究设计并构建了库容为2.0×109的大容量人源NaiveFab抗体库，并应用生物筛选法从中成功地筛选出一株抗Met人源抗体分子，该分子被命名为hFab-Met-1。hFab-Met-1的生物学特性及功能已被详细鉴定。 本文具体内容概括如下。　　实验一人源NaiveFab抗体库的构建。DNA测序结果表明大于86﹪(13/15)的克隆含有插入方向正确的全长Fab基因序列。 实验二人源NaiveFab抗体库的筛选及抗MetFab抗体片断的特征鉴定。结果显示，肿瘤部位的特异性造影可在注射后1小时时出现，最长可持续到24小时。在造影前期，同位素活性在肾脏高度聚集；在后期则主要集中在胸腺。总之，hFab-Met-1可与Met分子在体外和体内特异性结合，表明hFab-Met-1有希望作为一候选分子在肿瘤的临床诊断和治疗上发挥作用。

目录

EXPERIMENT PART 1、The Construction of Human Naive Fab Library

1Introduction

2Materials and Apparatus

3Methods

4Result

5Discussion

6Conclusion

Reference

EXPERIMENT PART II、Panning of Human Naive Fab Library and Characterizat ion of Ant i-Met Fab Fragment Generated from It

1Introduction

2Materials and Apparatus

3Methods

4Discussion

5Conclusion

Reference

致谢

REVIEW PART1、The Application of Engi neered Anti bodies for Cancer Diagnosis and Therapy

1Introduction

2Genetically Engineered Monoclonal Antibodies

3Alternative Strategies for Producing“Human”Antibodies

4Antibody fragments,novel design for diverse purposes

5Affinity maturation

Reference

REVIEW PART II、Molecular Targeting of the Met-HGF/SF Signaling Pathways：a Useful Treatment Strategy for Cancer

附录

1.Figure number 3 of experiment part Ⅰ.the sequence of 1 3 clones picked up from constructed Fab library.

2.Abbreviations

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