声明
List of commonly used abbreviations
1. Introduction
1.1 Differential miRNAs
1.2 Developmental hypothesis
2. Materials and methods
2.1 Chicken embryo incubation and tissue collection
2.2 Total RNA isolation and reverse transcription
2.3 Identification of differentially expressed miRNAs
2.4 Differentially expressed miRNAs and genes network construction
2.5 Analysis of miRNA quantification by RT-PCR
2.6 Determination of mRNA levels by RT-PCR
2.7 Bioinformatics analysis
2.8 Plasmid construction
2.9 Predict the target gene CPEB3 and FGFR3 mutant 3'UTR amplification primers
2.10 Cell culture and Luciferase reporter assays
2.11 Statistical analysis
3. Results
3.1 Analysis of Small RNAs
3.2 Identification of known and novel miRNA
3.3 miRNAs expression and differentially significant expressed miRNAs
3.4 Identifying mRNAs expression and differentially expressed mRNAs
3.5 Integrated analysis of differentially expressed genes and miRNAs
3.6 qPCR validation of sequencing data
3.7 Target genes prediction, GO and KEGG analysis
3.8 KEGG pathway enrichment analysis
3.9 Verification of the interaction between miRNA and target gene
4. Discussion
4.1 miRNA target validation
4.2 miRNA and mRNA interaction in skeletal muscle
5. Conclusion and future prospects
致谢
参考文献
Appendix
